What Is Ovagen? Dosage, Half-Life & How to Track It
Also known as: Glu-Asp-Leu · EDL · Khavinson GI bioregulator
Ovagen is an ultrashort synthetic tripeptide (Glu-Asp-Leu) studied as a GI- and liver-tissue bioregulator. Doses are tiny compared with most peptides — protocols run subcutaneously once daily starting around 10 mcg and titrating up to a 100–150 mcg maintenance range over roughly 16 weeks. The evidence is entirely preclinical — in-vitro cell studies, not human trials. Educational information only.
What Ovagen is and how it works
Ovagen is one of the shortest peptides in the Khavinson bioregulator family — just three amino acids, glutamic acid, aspartic acid, leucine (Glu-Asp-Leu, abbreviated EDL). It is studied as a tissue-selective regulator for the gastrointestinal tract and liver.
In-vitro work on renal cell cultures found that EDL increases cell proliferation and shifts expression of several aging-related genes — p16, p21, p53, and SIRT-6 — markers commonly used in senescence research. That is the entire evidentiary base: a cell-culture study, not an animal model or a human trial.
No controlled human trials exist for Ovagen. Anything beyond "it does something to these gene markers in a dish" is extrapolation, and the microgram-scale dosing protocol below is a community convention built by analogy with other bioregulators, not a clinically derived dose.
Typical dosing & reconstitution
Ovagen is reconstituted with bacteriostatic water — a 20 mg vial mixed with 2.0 mL gives 10 mg/mL, where one U-100 unit equals 100 mcg.
The doses themselves are unusually small for a peptide protocol, which is why the titration schedule moves in single-digit-microgram steps:
| Phase | Daily dose | Units (U-100) |
|---|---|---|
| Weeks 1–2 | 10 mcg | 0.1 units |
| Weeks 3–4 | 20 mcg | 0.2 units |
| Weeks 5–6 | 50 mcg | 0.5 units |
| Weeks 7–8 | 100 mcg | 1 unit |
| Weeks 9–16 | 100–150 mcg | 1–1.5 units |
Every dose in this protocol is under 1.5 units — a standard 100-unit insulin syringe simply can't read that precisely. A 30- or 50-unit syringe, with finer graduations per unit, is close to mandatory here, not optional.
Half-life & what it means for frequency
There is no published pharmacokinetic profile for Ovagen — no half-life, no clearance data. The once-daily, slow-titration schedule reflects how it is dosed in the available research and general bioregulator convention, not a measured blood curve.
Because the doses are so small to begin with, the bigger practical risk isn't frequency — it's measurement error. A syringe that's hard to read accurately at sub-1-unit volumes matters more here than almost anywhere else in this compound class.
What to track & possible side effects
There is essentially no human tolerability data for Ovagen — what's published is limited to cell-culture work, which doesn't speak to side effects in a person at all. Treat it as an unknown-safety-profile compound rather than one with a track record.
Given the 16-week titration and microgram dosing, what's worth logging is straightforward: exact dose and week, plus whatever GI or liver-related marker motivated trying it in the first place, so a slow ramp doesn't quietly drift off schedule.
How to track Ovagen in Stackeddd
Ovagen's microgram-scale dosing is where manual tracking falls apart fastest — log the exact dose and week so a 16-week titration doesn't drift. Run the reconstitution math first: at these volumes, syringe choice is the difference between a real dose and a guess.
Model it yourself · free, no account
Reconstitution Calculator
Model it yourself: enter your vial size and BAC water and the Peptides tab returns concentration and exact units for any dose — down to fractional units — the same math built into the Stackeddd app.
Related compounds
Related reading: Peptide reconstitution math: how much BAC water to add
Frequently asked questions
What is Ovagen?
Ovagen is a synthetic tripeptide (Glu-Asp-Leu, or EDL) from the Khavinson bioregulator family, studied as a gastrointestinal- and liver-tissue regulator. Evidence is limited to in-vitro cell-culture studies; it is not FDA-approved and has no human clinical trials.
What is Ovagen used for?
Cell-culture research links EDL to increased cell proliferation and changes in aging-related gene markers (p16, p21, p53, SIRT-6) in renal cells. There is no human data confirming these effects translate to a person.
How is Ovagen dosed?
Protocols use microgram-scale subcutaneous dosing once daily, titrating from about 10 mcg up to 100–150 mcg over roughly 16 weeks. A 20 mg vial reconstituted with 2.0 mL of bacteriostatic water gives 10 mg/mL.
Why are Ovagen doses so small compared with other peptides?
The community protocol scales the dose down to microgram amounts by analogy with the tiny quantities used in the underlying cell-culture research — there is no human dose-finding study establishing this range, so it should be treated as a cautious extrapolation, not a proven regimen.
This is educational information, not medical advice. Doses and protocols above reflect published references and community practice — protocol decisions belong with your prescribing physician. Generic names used throughout. How this reference is built & how the AI is tested →
STACKEDDD
Track it instead of guessing.
Doses, reconstitution, bloodwork, and an AI coach grounded in your own data — free to start, on the App Store and Google Play.