What Is DSIP? Dosage, Half-Life & How to Track It
Also known as: Delta Sleep-Inducing Peptide · DSIP Nonapeptide · WAGGDASGE
DSIP (Delta Sleep-Inducing Peptide) is a naturally occurring nine-amino-acid peptide first isolated from rabbit brain tissue, studied for its effect on delta-wave (deep) sleep and stress-hormone regulation. It is dosed subcutaneously once daily, in the evening, with typical amounts of 100–300 mcg, titrated up over several weeks. Its blood half-life is short — a matter of minutes — which is why it is timed right before bed rather than dosed earlier in the day. Educational information only.
What DSIP is and how it works
DSIP takes its name from its discovery: it was first isolated from rabbit brain tissue during studies on sleep induction, and it turns out to occur naturally in humans too — in cerebrospinal fluid and blood, at levels that appear to fluctuate with sleep stage. Despite the name, it is not a sedative the way a sleep drug is; it is a signaling peptide studied for its influence on sleep architecture — the pattern and depth of sleep stages across the night, not just whether you fall asleep.
The specific interest is delta-wave (slow-wave) sleep, the deepest, most physically restorative stage. DSIP is also studied for a second, related role: modulating the HPA axis — the stress-hormone system — by influencing cortisol and ACTH output, which is part of why it is framed as much as a stress-response peptide as a sleep one.
Human data is limited and decades old in places, with more recent research thin; DSIP is not FDA-approved and is not a substitute for treating an underlying sleep disorder. Persistent or unusual sleep issues are worth taking to a doctor rather than self-treating with a research peptide.
Typical dosing & reconstitution
DSIP is reconstituted from a lyophilized vial with bacteriostatic water and dosed once daily, roughly 30–60 minutes before bedtime. Typical protocols start around 100 mcg and titrate up to 250–300 mcg over the first month; doses above 300 mcg are anecdotal rather than clinically studied, since formal research mostly capped out near 300 mcg/day.
Two common reconstitution setups:
| Vial | BAC water | Concentration | 1 unit (U-100) ≈ |
|---|---|---|---|
| 5 mg | 3 mL | 1.67 mg/mL | 16.7 mcg |
| 10 mg | 3 mL | 3.33 mg/mL | 33.3 mcg |
At 1.67 mg/mL, a 200 mcg dose is 12 units on a U-100 insulin syringe. The reconstitution calculator works out the units for any vial size and water amount.
Half-life & what it means for frequency
DSIP clears from the blood quickly, on the order of minutes, similar to the other short peptides in this family. That is consistent with a single nightly dose rather than anything sustained — the point isn’t to keep a blood level elevated, it’s to deliver a signal around the time sleep architecture is being set for the night.
Because it clears fast and is timed to one part of the day, DSIP is one of the more forgiving peptides to log: there’s no accumulation to track across the week, just whether last night’s dose happened and when.
What to track & common side effects
DSIP is generally reported as well tolerated, with the most common complaints being vivid dreams, mild grogginess the next morning if dosed too early or too high, and occasional injection-site irritation. Because doses above the studied ~300 mcg range aren’t backed by formal data, going higher is a step further from what has actually been tested.
The useful things to track are simple but easy to lose without a log: the dose and injection time, and a subjective sleep-quality rating the next morning — the two data points that actually tell you whether the titration is working or just adding side effects.
How to track DSIP in Stackeddd
Log the nightly DSIP dose and timing so you can see whether an earlier or later injection changes how you feel the next morning. Pair it with a simple sleep-quality note — or your Health Connect / Apple Health sleep data — so the trend is visible instead of remembered. Run the reconstitution once so the units match the dose you intend.
Model it yourself · free, no account
Reconstitution Calculator
Model it yourself: enter your vial size and how much BAC water you added, and the Peptides tab returns the concentration and the exact insulin-syringe units for any dose — the same math built into the Stackeddd app.
Related compounds
Related reading: Peptide reconstitution math: how much BAC water to add · What peptides can you mix in the same syringe?
Frequently asked questions
What is DSIP used for?
DSIP is studied for its effect on delta-wave (deep) sleep and for modulating cortisol/ACTH as part of the stress-hormone (HPA axis) system. It is not a sedative and not FDA-approved; evidence is limited and largely older research.
What is the half-life of DSIP?
Short — on the order of minutes in the bloodstream — which is why it is dosed once, timed 30–60 minutes before bed, rather than spread across the day.
When should you inject DSIP?
In the evening, typically 30–60 minutes before bedtime, once daily. Dosing earlier in the day works against its studied purpose of influencing the sleep window.
How much DSIP should you take?
Typical protocols start around 100 mcg and titrate to 250–300 mcg over several weeks. Formal human studies mostly used doses up to about 300 mcg/day; higher amounts are anecdotal rather than clinically studied.
This is educational information, not medical advice. Doses and protocols above reflect published references and community practice — protocol decisions belong with your prescribing physician. Generic names used throughout. How this reference is built & how the AI is tested →
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